Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging.
Identifieur interne : 000F59 ( Main/Exploration ); précédent : 000F58; suivant : 000F60Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging.
Auteurs : RBID : pubmed:23010859English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Arsenicals, Indium, Polyethyleneimine, Silanes.
- analysis : Nanowires.
- methods : Optical Imaging.
- ultrastructure : Cell Membrane.
- HEK293 Cells, Humans, Microscopy, Confocal, Tissue Array Analysis.
Abstract
The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11 μm long and 3-7 μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.
DOI: 10.1088/0957-4484/23/41/415102
PubMed: 23010859
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging.</title><author><name sortKey="Berthing, Trine" uniqKey="Berthing T">Trine Berthing</name><affiliation wicri:level="1"><nlm:affiliation>Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen</wicri:regionArea></affiliation></author><author><name sortKey="Bonde, Sara" uniqKey="Bonde S">Sara Bonde</name></author><author><name sortKey="Rostgaard, Katrine R" uniqKey="Rostgaard K">Katrine R Rostgaard</name></author><author><name sortKey="Madsen, Morten Hannibal" uniqKey="Madsen M">Morten Hannibal Madsen</name></author><author><name sortKey="S Rensen, Claus B" uniqKey="S Rensen C">Claus B Sørensen</name></author><author><name sortKey="Nyg Rd, Jesper" uniqKey="Nyg Rd J">Jesper Nygård</name></author><author><name sortKey="Martinez, Karen L" uniqKey="Martinez K">Karen L Martinez</name></author></titleStmt><publicationStmt><date when="2012">2012</date><idno type="doi">10.1088/0957-4484/23/41/415102</idno><idno type="RBID">pubmed:23010859</idno><idno type="pmid">23010859</idno><idno type="wicri:Area/Main/Corpus">000A30</idno><idno type="wicri:Area/Main/Curation">000A30</idno><idno type="wicri:Area/Main/Exploration">000F59</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arsenicals (chemistry)</term><term>Cell Membrane (ultrastructure)</term><term>HEK293 Cells</term><term>Humans</term><term>Indium (chemistry)</term><term>Microscopy, Confocal</term><term>Nanowires (analysis)</term><term>Optical Imaging (methods)</term><term>Polyethyleneimine (chemistry)</term><term>Silanes (chemistry)</term><term>Tissue Array Analysis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arsenicals</term><term>Indium</term><term>Polyethyleneimine</term><term>Silanes</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Nanowires</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Optical Imaging</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Membrane</term></keywords><keywords scheme="MESH" xml:lang="en"><term>HEK293 Cells</term><term>Humans</term><term>Microscopy, Confocal</term><term>Tissue Array Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11 μm long and 3-7 μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23010859</PMID><DateCreated><Year>2012</Year><Month>09</Month><Day>28</Day></DateCreated><DateCompleted><Year>2013</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1361-6528</ISSN><JournalIssue CitedMedium="Internet"><Volume>23</Volume><Issue>41</Issue><PubDate><Year>2012</Year><Month>Oct</Month><Day>19</Day></PubDate></JournalIssue><Title>Nanotechnology</Title><ISOAbbreviation>Nanotechnology</ISOAbbreviation></Journal><ArticleTitle>Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging.</ArticleTitle><Pagination><MedlinePgn>415102</MedlinePgn></Pagination><Abstract><AbstractText>The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11 μm long and 3-7 μm spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berthing</LastName><ForeName>Trine</ForeName><Initials>T</Initials><Affiliation>Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark.</Affiliation></Author><Author ValidYN="Y"><LastName>Bonde</LastName><ForeName>Sara</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Rostgaard</LastName><ForeName>Katrine R</ForeName><Initials>KR</Initials></Author><Author ValidYN="Y"><LastName>Madsen</LastName><ForeName>Morten Hannibal</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Sørensen</LastName><ForeName>Claus B</ForeName><Initials>CB</Initials></Author><Author ValidYN="Y"><LastName>Nygård</LastName><ForeName>Jesper</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>Karen L</ForeName><Initials>KL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanotechnology</MedlineTA><NlmUniqueID>101241272</NlmUniqueID><ISSNLinking>0957-4484</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Arsenicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Silanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>1303-11-3</RegistryNumber><NameOfSubstance>indium arsenide</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-98-6</RegistryNumber><NameOfSubstance>Polyethyleneimine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Arsenicals</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">HEK293 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanowires</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Optical Imaging</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Polyethyleneimine</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Silanes</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tissue Array Analysis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2012</Year><Month>9</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1088/0957-4484/23/41/415102</ArticleId><ArticleId IdType="pubmed">23010859</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F59 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F59 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23010859
|texte= Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23010859" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |